Recent research on: “mesenchymal stem cells from bone marrow transplanted mounted on a scaffolding of Plasma Rich in Platelets and fibrin stimulates cartilage defects to completely heal.”
This study support our research and informs patients about using human stem cells from bone marrow to articular cartilage lesions. Articular cartilage covers the articulating surfaces and is more susceptible to injury compared to other types of cartilage.
Researchers at the School of Medicine, Cairo University and the University of Pittsburgh reported on the use of mesenchymal stem cells from bone marrow and scaffolding platelet-rich fibrin to heal cartilage defects in five patients
Why use stem cells from the bone marrow in Prolotherapy?
Articular cartilage has a limited capacity for repair and bone stimulation procedures in cases microfracture, osteochondral grafts as implants and autologous cartilage have had limited success in articular cartilage defects.
This study researchers chose mesenchymal stem cells from bone marrow because these have the capacity to differentiate into cartilage cells. In the case of these five patients it was extracted bone marrow from the iliac crest (hip bone).
Platelets were used as a scaffold because Platelets contain several growth factors that stimulate cartilage regeneration. The researchers expected that the biological effect of multiple growth factors in tissue regeneration was greater than that of a single growth factor.
Patients showed significant functional improvement. Two patients underwent arthroscopy and after transplantation showed almost normal articular cartilage. Three images of MRI (Magnetic Resonance Imaging) revealed postoperative complete healing and cartilage tissue congruent, while two MRIs of patients showed incomplete congruence in the cartilaginous tissue.
The researchers concluded that transplantation of autologous stem cells from bone marrow expanded linked to Plasma Rich in Platelets and fibrin shows great promise in the treatment of articular cartilage defects, particularly defects large (> 4 cm). 1 year positive clinical results support further randomized controlled trials of this treatment modality with more patients and longer follow-up periods.
We use the stem cells from bone marrow in a more direct way, nonsurgical regenerate cartilage
Prolotherapy cell with Marrow Aspiration involves direct (or also concentrated) bone marrow stem cells for to be used in the site of injury. Does the earlier study suggests that direct injections of bone marrow without cultivation expanded without the scaffolding could work? Possibly, but only time will tell to measures that advance the investigation. However, in our experience we have found that these stem cells act as large proliferating solutions within prolotherapy treatment. We use the bone marrow stem cells in conjunction with other solutions injections for treating large defects in joint areas labral and meniscus. Usually, patients are seen every 1-2 months. Most patients require 3-6 visits. The good news is that during the healing time, the patient can exercise and start getting back in top form! Treatments stem cell from bone marrow and adipose tissue derivatives they are gaining recognition and are happy to offer them as an option for the treatment of chronic wounds and sports injuries.
Research into the effectiveness of treatment protocols with stem cell injections
Research on the effectiveness of any treatment protocol is always in conflict and stem cell treatment does not escape this reality. Many times a patient points to his own dissatisfaction or clinical research and says: “… stem cell therapy does not work as well as advertised. Let’s look at the research:
“Osteoarthritis is a degenerative process They cartilage … No treatment is available to improve or reverse the process. Immunotherapy opened new horizons for the treatment of many incurable diseases. In this study four patients with knee osteoarthritis were selected for the study. they were 55, 57, 65 and 54, and had osteoarthritis of the knee that was moderate to severe. After written consent signed, 30 ml of bone marrow were taken and cultured to grow the number of cells mesenchymal (MSC). After having enough MSCs in culture (4-5 weeks) and taking into account all safety measures, the cells were injected into each patient’s knee.
Walking time for the pain appeared improved in three patients and remained unchanged for one. On physical examination, the improvement was mainly for crepitus (sound or click). The improvement was somewhat lower in amplitude range of motion.
The results were good, but not excellent, we believe that improvements in technique may improve results.
Improve the technique of combining cells with mothers Therapy Platelet Rich Plasma (PRP)
We agree that stem cell therapy has benefits, but can not provide a complete cure. That is why the use of therapy platelet-rich plasma in combination with stem cell therapy is recommended. The previous study cultured stem cells. In our opinion the stem cell therapy is more effective if the stem cells are given better guidance. This is where the growth factors that are in the blood platelets can be very effective. Platelets help stem cells at various jobs, including differentiation and then helping in differentiated cells build extracellular matrix to repair the injured tissue.
Platelet rich plasma contains a myriad of substances that stimulate healing:
to. Growth factor, platelet derived (PDGF) attracting cells of the immune system and stimulates area them to proliferate. It has been shown to enhance the healing of ligaments and tendons.
b. Transforming Factor-8 (TGF-8) affecting the main types of cells involved in healing. effects similar to PDGF.
c. Vascular Endothelial Growth Factor (VEGF). It helps the formation of new blood vessels, which increases vascularization in injured areas.
d. Fibroblast growth factor (FGF) promotes the growth of cells involved in the formation of collagen and cartilage.
Numerous studies have shown that PRP improves the effects of treatment with stem cells as the study noted above: “Therapy platelet rich plasma (PRP) technique enhances and improves results.
The goal of stem cell therapy – Prolotherapy cell
Our ultimate goal with all forms of prolotherapy is getting patients back to doing things they want to do without pain. We hope that stem cell therapy (Prolotherapy Cell) will form tissues that are functional, structural and mechanically the same, if not better than damaged tissue. It is difficult to prove the above statement, because we can not sacrifice human beings after prolotherapy sessions to see if the tissue looks and acts normally. We can, however, report that most of our patients receiving stem cell therapy along with standard Prolotherapy Hackett-Hemwall return to their activities and have drastically reduced pain levels using this comprehensive approach.
Center Prolotherapy and Regenerative Medicine Intensive, we have over 90% effectiveness in significant improvement and / or cure pain who have come up with major injuries to his knees, shoulders and hips.
Using Stem Cells to repair tendon or ligament injuries.
Not all lesions require the use of stem cells to cure. In our center, the success rate with the Traditional Prolotherapy (Hackett-Hemwall) is around 90% in most of the wide range of patients we see.
However, for cases of very aggressive advanced arthritis, tendonitis (tendon degeneration), meniscal tears, labral tears (or impeller articular hip or shoulder) injuries where there rubbing bone to bone, or injury where there is little cellularity, we can choose to use injections of stem cells in combination with the standard Prolotherapy with dextrose to strengthen and stabilize the support structures around the injury and to repair the meniscus and better recovery and / or cure.